Spontaneous pre-Descemet's membrane corneal opacities in rabbits.
نویسندگان
چکیده
310 There are few reports of spontaneous ocular changes in laboratory rabbits (1), which when detected may complicate interpretation of ocular results in toxicity testing. The corneal changes that have been described in this species include anterior corneal dystrophy in American Dutch belted rabbits (2) and corneal epithelial dystrophy in New Zealand White (NZW) rabbits (3, 4). In addition, diet-induced corneas lipidosis has been described in NZW rabbits (5). In humans, a number of corneal dystrophies are recognized, including that of pre-Descemet’s membrane (6–8). To the authors’ knowledge, pre-Descemet’s membrane dystrophy has not been reported in laboratory animals. We describe a pre-Descemet’s membrane corneal abnormality in rabbits and give some insights into its etiopathogenesis. Seventy-six albino (43 males and 33 females) NZW (SPF NZW A 1077 INRA) rabbits, 2 to 4 months old, were obtained from a commercial supplier (Centre Lago, Vonnas, France). They were housed individually in stainless steel cages, fed a commercial laboratory diet (UAR 112 C Laboratory Chow; UAR, Villemoisson sur Orge, France), and provided tap water ad libitum. Rabbits were kept at a temperature of 19 6 18C and 30 to 70% relative humidity. There were approximately 15 air changes/h and a 12/12-h light/ dark cycle. The animal facility has animal care and use programs that are accredited by AAALAC, International; the Animal Care Program operates in accordance with current standards. Eye examinations were performed, using an indirect ophthalmoscope (Welch Allyn, Inc., Skaneateles Falls, N.Y.) with interposition of a 28-diopter Nikon lens and a hand-held slit lamp biomicroscope (Kowa Co., Ltd., Tokyo, Japan). Before examination, pupils were dilated by use of 0.5% tropicamide (Mydriaticum; MSD-Chibret, Paris, France). Ocular examinations were performed after rabbits had been in the laboratory for at least 2 weeks. Eight rabbits were examined for ocular changes every 1 to 2 months for 6 months. After the last ocular examination, rabbits were euthanized by intravenous administration of an overdose of 6% pentobarbital (Pentobarbital Sodique; Sanofi Sante Animale, Libourne, France). Eyes were fixed in Zenker’s fixative, processed in routine manner, embedded in paraffin, and cut in 5-mm sections. Sections were stained with hematoxylin and eosin (H&E). Eyes from two rabbits with multifocal and bilateral corneal opacities were immersed in 4F1G (4% formaldehyde + 1% glutaraldehyde) fixative solution. Blocks of approximately 2 x 2 mm2 were
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عنوان ژورنال:
- Laboratory animal science
دوره 48 3 شماره
صفحات -
تاریخ انتشار 1998